Thirty days of HFD feeding results in higher percent weight gain and epididymal fat tissue than LFD. Before switching to either the HFD or the LFD, the absolute weight (A) of the LFD group and the HFD group were similar (day 0). No significant differences were observed during the dietary intervention, or at time of sacrifice (day 30) between animals on the LFD and HFD (B). However, (C) animals in the HFD group showed a larger increase in percent weight gained at several time points during the intervention. (D) Animals fed with HFD consumed more kilocalories than LFD during week 1, week 2 and week 4 (n = 6/group). (E) Both groups showed no significant differences in feeding efficiency percent during the four weeks (n = 6/group). (F) Additionally, the epididymal fat pads were found to be significantly heavier in the HFD group compared to LFD. Data represented as mean ± standard error of the mean; n = 14/group except where indicated otherwise. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.