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. 2022 Oct 27;8(11):e11194. doi: 10.1016/j.heliyon.2022.e11194

Figure 5.

Figure 5

HFD rats have increased Bacteroides and decreased expression of tight junction proteins in the distal colon. Alpha diversity shows no difference between HFD and LFD group (A) in PD Whole Tree, (B) Chao1 box plot, (C) Shannon index & Pielou index (p > 0.05; n = 6/group). (D) However, differences in beta diversity were found between the LFD and HFD animals (p < 0.05; n = 6/group). (G& H) Bacterial abundance was significantly different at both family and genus level, however (E&F) Firmicutes/Bacteroidetes abundance and ratio is similar in both groups (n = 6/group). (G & H) Bacteroides was significantly increased in HFD whereas S24-7 family and genus levels were significantly decreased. (I) LPS levels in the blood were higher in the HFD group compared with the LFD group (LFD, n = 10; HFD, n = 11). (J) Analysis of tight junction proteins revealed lower mRNA expression of claudin-5 (LFD, n = 11; HFD, n = 13) and occludin in the HFD compared with LFD (LFD, n = 10; HFD, n = 11). (K) Significant correlations were found between % Bacteroides vs Claudin-5 mRNA, and % S24-7 vs Claudin-5 mRNA. Data are represented as mean ± standard error of the mean (n = 11/group). P values ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.