Table 1.
Experimental design and enzymes activities (U/mL) using Plackett–Burman factorial design.
| Run | Temperature (°C) | pH | Time (h) | MgSO4 (%w/v) | NaCl (%w/v) | Fe2SO4 (%w/v) | (NH4)2SO4 (%w/v) | Pectinase (U/ml) | PGase (U/ml) | Xylanase (U/ml) | Cellulase (U/ml) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 40 | 6 | 96 | 0.02 | 0.1 | 0.01 | 0.5 | 6.54 | 4.98 | 3.39 | 3.10 |
| 2 | 40 | 9 | 12 | 0.05 | 0.1 | 0.01 | 0.1 | 2.25 | 3.72 | 1.46 | 0.00 |
| 3 | 30 | 9 | 96 | 0.02 | 0.5 | 0.01 | 0.1 | 5.01 | 5.25 | 5.84 | 0.00 |
| 4 | 40 | 6 | 96 | 0.05 | 0.1 | 0.05 | 0.1 | 5.91 | 8.01 | 6.26 | 7.26 |
| 5 | 40 | 9 | 12 | 0.05 | 0.5 | 0.01 | 0.5 | 1.99 | 3.60 | 0.71 | 0.00 |
| 6 | 40 | 9 | 96 | 0.02 | 0.5 | 0.05 | 0.1 | 3.73 | 1.85 | 0.00 | 0.00 |
| 7 | 30 | 9 | 96 | 0.05 | 0.1 | 0.05 | 0.5 | 6.60 | 5.60 | 8.25 | 6.96 |
| 8 | 30 | 6 | 96 | 0.05 | 0.5 | 0.01 | 0.5 | 5.69 | 5.49 | 7.25 | 4.15 |
| 9 | 30 | 6 | 12 | 0.05 | 0.5 | 0.05 | 0.1 | 2.39 | 3.00 | 1.83 | 0.00 |
| 10 | 40 | 6 | 12 | 0.02 | 0.5 | 0.05 | 0.5 | 2.03 | 2.75 | 1.19 | 0.00 |
| 11 | 30 | 9 | 12 | 0.02 | 0.1 | 0.05 | 0.5 | 2.34 | 2.50 | 2.60 | 0.00 |
| 12 | 30 | 6 | 12 | 0.02 | 0.1 | 0.01 | 0.1 | 1.51 | 1.89 | 0.71 | 0.00 |
| 13 | 35 | 7.5 | 54 | 0.035 | 0.3 | 0.03 | 0.3 | 7.42 | 7.13 | 8.13 | 6.00 |
| 14 | 35 | 7.5 | 54 | 0.035 | 0.3 | 0.03 | 0.3 | 6.15 | 6.78 | 5.77 | 5.47 |
| 15 | 35 | 7.5 | 54 | 0.035 | 0.3 | 0.03 | 0.3 | 5.92 | 5.71 | 5.52 | 6.88 |