Table 2.
Box–Behnken Design for enzymes production by using orange peel as the substrate [−1, 0, and +1 are the codes for the variables].
| Run | Time (h) | Orange peel (% w/v) | Inoculum volume (% v/v) | Pectinase (U/ml) | PGase (U/ml) | Xylanase (U/ml) | Cellulase (U/ml) | ||
|---|---|---|---|---|---|---|---|---|---|
| Observed | Predicted | Observed | Observed | Predicted | Observed | ||||
| 1 | 12 (−1) | 0.5 (−1) | 2 (0) | 1.71 | 2.60 | 1.25 | 0.96 | 1.27 | 0.00 |
| 2 | 36 (+1) | 0.5 (−1) | 2 (0) | 7.41 | 8.33 | 5.65 | 8.59 | 8.41 | 0.00 |
| 3 | 12 (−1) | 1.5 (+1) | 2 (0) | 4.58 | 3.66 | 7.98 | 1.03 | 1.21 | 13.68 |
| 4 | 36 ( + 1) |
1.5 (+1) | 2 (0) | 6.53 | 5.64 | 6.12 | 7.09 | 6.77 | 17.22 |
| 5 | 12 (−1) | 1 (0) | 1 (−1) | 2.34 | 2.21 | 0.16 | 2.67 | 2.52 | 0.00 |
| 6 | 36 (+1) | 1 (0) | 1 (−1) | 5.52 | 5.37 | 5.87 | 8.68 | 9.02 | 8.8 |
| 7 | 12 (−1) | 1 (0) | 3 (+1) | 1.01 | 1.16 | 1.48 | 0.00 | 0.00 | 0.00 |
| 8 | 36 (+1) | 1 (0) | 3 (+1) | 5.57 | 5.70 | 7.27 | 5.72 | 5.87 | 13.69 |
| 9 | 24 (0) | 0.5 (−1) | 1 (−1) | 5.24 | 4.47 | 8.24 | 6.57 | 6.41 | 7.04 |
| 10 | 24 (0) | 1.5 (+1) | 1 (−1) | 4.49 | 5.54 | 4.62 | 8.23 | 8.20 | 17.34 |
| 11 | 24 (0) | 0.5 (−1) | 3 (+1) | 7.04 | 5.99 | 7.43 | 6.03 | 6.05 | 7.51 |
| 12 | 24 (0) | 1.5 (+1) | 3 (+1) | 2.54 | 3.30 | 0.11 | 2.38 | 2.54 | 0.00 |
| 13 | 24 (0) | 1 (0) | 2 (0) | 10.62 | 9.49 | 13.83 | 16.79 | 16.27 | 19.54 |
| 14 | 24 (0) | 1 (0) | 2 (0) | 8.15 | 9.49 | 10.66 | 15.75 | 16.27 | 17.90 |
| 15 | 24 (0) | 1 (0) | 2 (0) | 9.69 | 9.49 | 11.25 | 16.27 | 16.27 | 18.72 |