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. 2022 Nov 1;13:1024672. doi: 10.3389/fmicb.2022.1024672

Table 2.

Variations of the relative abundance of genes related to microbial biogeochemical cycling over different weathering times (mean relative abundance >1%).

Gene function category Relative abundance % (mean abundance) HmV and normality Slope SE R 2 p-param Adj R2 p-perm p-sign
S Cycle 0.49% (30566) Null −2.58E-04 1.47E-04 0.16 0.099 Null Null Null
N Cycle 0.95% (59035) No, HtV 8.73E-04 3.31E-04 0.30 0.018* 0.26 0.035 0.035*
C Cycle 2.34% (146672) No, HtV 1.38E-03 3.85E-04 0.44 0.003** 0.41 0.012 0.012*
C Fixation 76.09% (111643) Null 1.11E+00 5.51E-01 0.20 0.060 Null Null Null
C Degradation 12.70% (18588) No, HtV −1.51E+00 4.83E-01 0.38 0.007** 0.34 0.007 0.007**
Methane Metabolism 11.21% (16440) Null 3.97E-01 3.43E-01 0.08 0.265 Null Null Null
ANRA 6.65% (3911) Null −1.44E-02 2.20E-02 0.13 0.523 Null Null Null
DNRA 4.36% (2592) Null 1.29E-02 1.20E-02 0.12 0.302 Null Null Null
N Fixation 0.21% (122) Null 1.96E-04 4.79E-03 0.06 0.968 Null Null Null
N Transport 5.10% (2990) Null 1.75E-02 2.93E-02 0.17 0.560 Null Null Null
Denitrification 1.39% (804) Yes −3.27E-02 1.36E-02 0.55 0.002* 0.49 0.002 0.002**
Nitrification 0.65% (368) Yes −2.14E-02 8.12E-03 0.60 <0.001*** 0.55 <0.001 <0.001***
ONM 82.14% (48528) Null 2.15E-02 3.89E-02 0.03 0.588 Null Null Null
ASR 93.87% (28648) Yes 2.16E-02 8.26E-03 0.30 0.019* 0.26 0.019 0.019*
DSR 6.67% (2019) Null 7.26E-04 4.52E-03 0.00 0.874 Null Null Null
SOX 5.47% (1713) Yes −2.16E-02 7.60E-03 0.34 0.012* 0.29 0.012 0.012*
Hemicellulose 27.50% (5140) Null 2.19E-01 7.66E-01 0.01 0.779 Null Null Null
Starch 41.47% (7620) Yes 2.99E+00 1.24E+00 0.27 0.028* 0.22 0.028 0.028*
Aromatics 1.00% (181) Yes 2.74E-01 1.08E-01 0.29 0.021* 0.24 0.021 0.021*
Chintin 8.04% (1532) Null −1.07E+00 7.26E-01 0.12 0.159 Null Null Null
Lignin 0.03% (6) Null −2.60E-02 1.35E-02 0.19 0.072 Null Null Null
Cellulose 21.97% (4109) Yes −2.38E+00 1.01E+00 0.26 0.031* 0.21 0.031 0.031*
3HP/4HB 23.86% (26699) Yes −1.12E-02 3.96E-03 0.34 0.012* 0.29 0.012 0.012*
3HP 25.78% (28819) Yes −1.34E-02 3.73E-03 0.45 0.002** 0.41 0.002 0.002**
DC/4HB 36.16% (40311) No, HtV 2.05E-02 7.41E-03 0.32 0.014* 0.28 0.029 0.029*
rTCA 45.55% (50766) No, HtV 1.88E-02 7.46E-03 0.28 0.023* 0.24 0.046 0.046*
rAcCoA 10.89% (12227) Yes 3.27E-02 1.06E-02 0.39 0.008** 0.31 0.008 0.008**
Calvin cycle 27.87% (31093) Null −3.55E-01 2.60E-01 0.10 0.191 Null Null Null

The relative abundances of gene function category were averaged for each site (n = 18). The variance explained (R2), regression slope, and parametric p-value (p-param) of the linear regression, normality test, homogeneity test for variance, and permutation test p value (p-perm) with different weathering times were shown in the table (* indicates p < 0.05, ** indicates p < 0.01, and ***indicates p < 0.001). HmV, homogeneity of variance; HtV, heterogeneity of variance; p-sign, significance value of p.