FIGURE 6.

Experimental validation of representative biomarkers. (A) Western blot confirmed BGN and THBS2 stable knockdown in colon cancer cells constructed by plasmids containing BGN and THBS2‐targeting shRNA. (B) CCK8 assay showed that knockdown of the expression levels of BGN reduced the proliferation ability of colon cancer cell lines. (C) Colony formation assay showed that knockdown of the expression levels of BGN reduced the ability of colony formation of colon cancer cell lines. (D) Differences in migration and invasion ability of cells after knockdown of BGN and THBS2 expression levels, and the migration and invasion ability of the cells were both decreased when these two genes were knocked down. (E and F) Three proteins during epithelial‐mesenchymal transition (EMT) (Snail, Vimentin and N‐cadherin) were decreased by knockdown the expression of BGN and THBS2. (G) BGN and THBS2 may inhibit colon cancer cell migration and invasion by participating in the regulation of EMT process