Figure 3.

Myeloid follistatin-like protein 1 (FSTL1) deficiency attenuates inflammation in mice fibrotic livers. (A) Messenger RNA (mRNA) expression of tumour necrosis factor (TNF)-α, interleukin (IL)-1β and IL-10 was quantified in liver tissues; (B) serum TNF-α, IL-1β and IL-10 were measured by ELISA. (C) F4/80, (D) CD11b and (E) Ly6G were detected by immunofluorescence, and quantified as numbers of positive cells per high power field (HPF). Data were presented as mean±SEM; n=6–8 per group; scale bars, 50 µm; *p<0.05, **p<0.01, ***p<0.001. BDL, bile duct ligation; CCl₄, carbon tetrachloride; MCD, methionine-deficient and choline-deficient diet.