Figure 6.

Follistatin-like protein 1 (FSTL1) promotes pyruvate kinase M2 (PKM2) phosphorylation and nuclear translocation in macrophage of fibrotic liver tissues. Human data (A–B) and mice data (C–D): (A) immunofluorescence staining of FSTL1 (upper panels) or PKM2 (lower panels), CD68 and DAPI in human liver tissues; (B) expressions of PKM2 and FSTL1 were analysed by western blot (WB) analysis in cytoplasm and nucleus of human livers; (C) immunofluorescence staining of PKM2, CD68 and DAPI in mice fibrotic livers; (D) expression of PKM2 and p-PKM2 were determined by WB analysis in cytoplasm and nucleus of fibrotic livers. Scale bars, 20 μm. BDL, bile duct ligation; CCl₄, carbon tetrachloride; MCD, methionine-deficient and choline-deficient diet.