Figure 4.

Diarrheagenic MNV1 and WU23 can infect CD300lf^lo cells in vitro while attenuated CR6 cannot. (A, B) Duplicate wells of BV2 cells (A) or M12 cells (B) were infected with MNV1, CR6, or WU23 at MOI 0.05. Virus titers were determined by TCID₅₀ assay at the indicated timepoints on culture supernatants. P values were determined by ANOVA using Tukey’s multiple comparisons test. (C) Duplicate wells of M12 cells were infected with MNV1, CR6, or WU23 at MOI 10 or MOI 40 as indicated. Virus titers were determined by TCID₅₀ assay at the indicated timepoints. (D) CD300lf was overexpressed in M12 cells by lentivirus transduction. Duplicate wells of M12 cells or CD300lf-overexpressing M12 cells were infected with MNV1, CR6, or WU23, at MOI 5. Virus titers were determined by TCID₅₀ assay at 1 dpi. P values were determined using ANOVA with Sidak’s multiple comparisons test. At least three independent experiments were performed for all experiments. ****P < 0.0001.