Figure 8.

The hypervariable domain of VP1 regulates infection of CD300lf^lo cells and sensitivity to environmentally triggered infectivity enhancement. (A) Duplicate wells of M12 cells were infected with MNV1, CR6, WU23, or the indicated chimeric virus at MOI 0.05. Virus titers at 1 dpi were determined by TCID₅₀ assay on culture supernatants. All infections were repeated four times. P values were determined using ANOVA with Dunnett’s multiple comparisons test using CR6 as the control group. (B) BV2 cells were infected with 100 TCID₅₀ units per well of MNV1, WU23, CR6, CR6^VP1.MNV1, CR6^VP1.WU23, or CR6^P2VP1.WU23 in neutral PBS (pH 7.6) or PBS at pH 6.7. Virus titers were determined by plaque assay and normalized to viral titers following infection in media. P values were determined using ANOVA with Dunnett’s multiple comparisons test using CR6 as the control group. The experiment was repeated two times. *P < 0.05, **P < 0.01, ****P < 0.0001.