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. 2022 Nov 1;10:965382. doi: 10.3389/fcell.2022.965382

FIGURE 5.

FIGURE 5

Quantitative immunostaining of human skin sections for phalloidin. (A,B,F,G) Phalloidin labeling (white) for the (A) male proximal, the (B) male distal, the (F) female proximal, and the (G) female distal samples of the four age groups (20s, 40s, 60s, and 70s). (C,D,H,I) Quantitative analysis confirmed these results for each respective data set (x-fold normalized to average of 20s group) (3 images per individual, ≤ 20 axons per image, (C) n = 51 individuals in proximal male, (D) n = 27 in distal male, (H) n = 21 in proximal female, (I) n = 20 in distal female data). (E,J) Phalloidin intensity ratio between the male and female proximal and distal samples allowed to compare both sample sets. Scale bars in (A,B,F,G) are 10 µm. Points in graphs (C,D,H,I) represent on individuals. Data represent mean ± SEM. Kruskal–Wallis test with Dunn’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.