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Scheme of the plasmid construction for making Δsrf mutant in B amyloliquefaciens Ba01. Primers JC1718/1719 and JC1720/1721 were used to amplify 5′ and 3′ NCR sequences of srf gene cluster of B amyloliquefaciens Ba01, respectively, and fused together by fusion PCR using primers JC1718/1721. After enzyme digestions the ~2 kb cassette was ligated to pMiniMAD2 in order to generate disruption plasmid pRY1.
