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. 2022 Nov 1;13:1016776. doi: 10.3389/fimmu.2022.1016776

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Copyright © 2022 Lou, Peng, Wang, Wang, Du, Zhang, Zheng, Liu and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The proportion and phenotype of MDSCs after cryo-thermal therapy. (A) The proportion of splenic MDSCs on day 21 after cryo-thermal therapy. (B, C) The expression levels of CD86 and MHC-II (B), CD11c, and F4/80 (C) on MDSCs from tumor-bearing control (gray) and cryo-thermal therapy (solid line) were detected by flow cytometry. (D) The expression levels of functional molecules of MDSC were detected by qRT-PCR. *p < 0.05, ***p < 0.001. n = 4 for each group. MDSCs, myeloid-derived suppressive cells.