REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CD31 (BV421)	Biolegend	303124; RRID:AB_2563810
Anti-CD45 (BV421)	Biolegend	304031; RRID:AB_10900423
Anti-PDPN (PE-Cy7)	Biolegend	337013; RRID:AB_2563367
Anti-KRT5 (AF-488)	Abcam	193894; RRID:AB_2893023
Anti-Ki67	Thermo Fisher Scientific	RM-9106; RRID:AB_2341197
Anti-MUC5AC	Sigma-Aldrich	M5293; RRID:AB_2146985
Anti-TP63	Abcam	ab735; RRID:AB_305870
Anti-FOXJ1	eBioscience	14-9965-80
Biological samples
Human tracheobronchial blocks	This study
Chemicals, peptides, and recombinant proteins
CellFIX	BD Biosciences	340181
Permeabilization Buffer	Life Technologies	C10419
jetPEI	Polyplus Transfection	101–01N
PEG-it Virus Precipitation Solution	System Biosciences	LV810A-1
Zombie Violet Fixable Viability Kit	Biolegend	423113
Y-27632	Cambridge Bioscience	Y1000
Adenine	Sigma-Aldrich	A2786
Hydrocortisone	Sigma-Aldrich	H0888
EGF	Sino Biological	10605
Insulin	Sigma-Aldrich	I6634
Cholera Toxin	Sigma-Aldrich	C8052
T3	Sigma-Aldrich	T5516
Rat Tail Collagen I	BD Biosciences	354236
Critical commercial assays
PureLink HiPure Plasmid Maxiprep Kit	Thermo Fisher Scientific	K210006
Click-iT EdU Staining Kit (Alexa Fluor 488)	Life Technologies
Deposited data
RNA sequencing data	GEO	GEO: GSE148818
Experimental models: Cell lines
Human: primary tracheobronchial epithelial cell cultures	This study	N/A
Human: 293T cells	Available from ATCC	CRL-3216
Mouse: 3T3-J2 mouse embryonic fibroblasts	Fiona Watt (King’s College London)
Oligonucleotides
Puromycin Copy Number (Custom Taqman Probes)	Taqman	4331348
Recombinant DNA
Plasmid: pCDH-EF1-copGFP-T2A-Puro	Addgene	#72263
Plasmid: pCDH-CMV-mCherry-T2A-Puro	Addgene	#72264
Plasmid: pMDLg/pRRE	Addgene	#12251
Plasmid: pRSV-Rev	Addgene	#12253
Plasmid: pMD2.G	Addgene	#12259