Table 2.
Primers used for real time polymerase chain reaction (RT-PCR) analysis.
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| IL-1β | TGGAGAGTGTGGATCCCAAG | GGTGCTGATGTACCAGTTGG |
| IL-6 | CTTGGGACTGATGCTGGTGA | TTGGGAGTGGTATCCTCTGTGA |
| IL-1Ra | CTCCAGCTGGAGGAAGTTAAC | CTGACTCAAAGCTGGTGGTG |
| TGF-β1 | CAGTACAGCAAGGTCCTTGC | ACGTAGTAGACGATGGGCAG |
| VEGF | CTACCTCCACCATGCCAAGT | CACACAGGATGGCTTGAAGA |
| bFGF | AAAAGGCAAGATGCAGGAGA | TTTTGCAGCCTTACCCAATC |
| FLT1 | GGGCTGAAACCATGTGCAAG | GCCAAAGATGCACTCCTCCT |
| KDR | GGCATGGGGTCTGTTCTGAA | TTGGCCAGGAGACACGTAAC |
| RUNX-2 | TGGTTCTGTCATGGCGGGTA | TCTCAGATCGTTGAACCTTGCTA |
| OPN | ACGCCGACCAAGGAAAACTC | GTCCATAAACCACACTATCACCTCG |
| OCN | CATGAGAGCCCTCACA | AGAGCGACACCCTAGAC |
| GAPDH | ATCCCATCACCATCTTCC | GAGTCCTTCCACGATACCA |