REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli: Strain OP50	CGC	RRID:WB-STRAIN:WBStrain00041969
Chemicals, peptides, and recombinant proteins
Ethyl methanesulfonate 99%	Fisher Scientific	AAA1293806
SU-8 Photoresist	Kayaku Advanced Materials	Y111077 0500L1GL
1-methoxy-2-propanol acetate	VWR (Avantor Performance Materials US)	JT6343-5
1H,1H,2H,2H-Perfluorooctyltrichlorosilane	Fisher Scientific	AAL1660609
Sylgard 184 Kit	Ellsworth Adhesives	184 SIL ELAST KIT 3.9KG
Critical commercial assays
Qiagen DNeasy Blood and Tissue Kit	Qiagen	69504
Experimental models: Organisms/strains
C. elegans: Strain DCD214 [myo-2p::tagRFP::pab-1]	CGC	RRID:WB-STRAIN: WBStrain00005606
C. elegans: Strain PS3551 [hsf-1(sy411)]	CGC	RRID:WB-STRAIN: WBStrain00030901
C. elegans: Strain KU2 [jkk1(km2)]	CGC	RRID:WB-STRAIN: WBStrain00024034
C. elegans: Strain VS24 [kat-1(tm1037)]	CGC	RRID:WB-STRAIN: WBStrain00040200
Software and algorithms
ImageJ	NIH	RRID: SCR_003070
OASIS 2	Han et al.41	https://sbi.postech.ac.kr/oasis/
AutoCAD	AutoDesk	https://www.autodesk.com/products/autocad/overview
Trimmomatic version 0.39	Bolger et al.42	RRID:SCR_011848
Picard (2.27.4),	Broad Institute	https://broadinstitute.github.io/picard/
GATK (Genome Analysis Toolkit, version 4.2.6.1)	Broad Institute	https://gatk.broadinstitute.org/hc/en-us
Microfluidic Imaging and Screening Software	This publication	github.com/asanmiguel/AggregationScreening
Aggregation Image Analysis Software	This publication	github.com/asanmiguel/AggregationScreening