Fig. 4.

Lack of effects of Rho G, a known regulator of P-Rex1-Rac1 module, on GSIS in INS-1 832/13 cells. Panel A: Lysates from INS-1 832/13 cells, rats and human islets were analyzed for RhoG protein expression by Western blot analysis. Actin was used as loading control. Panel B: INS-1 832/13 cells were transfected with Con-si or RhoG-si as described in Methods. Cell lysates were analyzed by Western blotting for the expression of RhoG. Actin was used as loading control. A representative blot from three independent studies is shown here. Panel C: Following 48 hours of transfection, cells were subjected to overnight starvation and then were treated with LG (2.5 mM) or HG (20 mM) for 45 mins. Insulin secretion in the media was determined as described in the Methods section. Data are mean ± SD from three experiments. The data are expressed as fold change relative to LG-Mock. (* p< 0.05). Comparisons shown: a - significant compared with LG-treated mock; b – significant compared with LG-treated Con-si; c - significant compared with LG-treated RhoG-si.