TABLE 1.
Effect of anti-CD14 MAb 18E12 on production of TNF and IL-10 by monocytes stimulated by LPS, H. influenzae, or S. pneumoniaea
| Stimulus | Concn/ml | Mean concn (pg/ml) ± SD (n = 3–6)
|
|||
|---|---|---|---|---|---|
| TNF
|
IL-10
|
||||
| Control MAb | 18E12 | Control MAb | 18E12 | ||
| LPS | 10 ng | 515 ± 338 | 272 ± 230b (47c) | 753 ± 362 | 591 ± 323b (22) |
| 5 ng | 1,021 ± 1,061 | 309 ± 340b (70) | 678 ± 348 | 329 ± 121b (52) | |
| 1 ng | 940 ± 961 | 88 ± 96b (91) | 350 ± 149 | 79 ± 62b (77) | |
| H. influenzae | 106 | 1,009 ± 771 | 563 ± 472b (44) | 1,026 ± 864 | 770 ± 664b (25) |
| 5 × 105 | 643 ± 607 | 384 ± 301b (40) | 898 ± 772 | 663 ± 462b (26) | |
| 105 | 223 ± 186 | 129 ± 115b (42) | 231 ± 214 | 34 ± 27b (85) | |
| S. pneumoniae | 106 | 932 ± 1,133 | 582 ± 805b (38) | 792 ± 347 | 630 ± 356 (21) |
| 5 × 105 | 742 ± 792 | 638 ± 961 (14) | 1,048 ± 698 | 441 ± 243b (58) | |
a
Adherent monocytes were stimulated for 24 h with LPS, heat-killed H. influenzae, or heat-killed S. pneumoniae in the presence of MAb 18E12 or the corresponding control MAb.
b
Cytokine production by LPS- or bacterium-stimulated monocytes in the presence of anti-CD14 MAb significantly (P < 0.05) less than with the corresponding control MAb.
c
Percent inhibition.