Table 4.

Incubation under the appropriate growth conditions.

Organisms	Selective medium agar	Atmosphere	Incubation
Lactobacillus gasseri	Human Blood Bilayer Tween	Anaerobic	35 ± 2 °C, 48 ± 2 h
Gardnerella vaginalis	Human Blood Bilayer Tween	Anaerobic	35 ± 2 °C, 48 ± 2 h
Prevotella bivia	Laked Blood Kanamycin-Vancomycin	Anaerobic	35 ± 2 °C, 48 ± 2 h
Escherichia coli	MacConkey Medium	Aerobic	35 ± 2 °C, 24 ± 2 h
Staphylococcus aureus	Mannitol Salt Agar	Aerobic	35 ± 2 °C, 48 ± 2 h
Candida albicans	Inhibitory Mould Agar with chloramphenicol and gentamycin	Aerobic	35 ± 2 °C, 48 ± 2 h

All selective media were obtained from Remel Microbiology Products (Fisher Thermo Scientific, USA).

Before plating on Human Blood Bilayer Tween, the respective aliquots were treated with 2 μg Lysostaphin (Sigma–Aldrich, USA) for 10 min at 37 °C. Pilot studies demonstrated that this procedure prevented the overgrowth of S. aureus on HBT without affecting growth of G. vaginalis and L. gasseri (data not shown).