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. 2022 Oct 28;66:101624. doi: 10.1016/j.molmet.2022.101624

Figure 2.

Figure 2

Time course of EEC differentiation after FoxO1 ablation. (A–D) Time course analysis of FoxO1 (A), Ngn3 (B), ChgA (C), and Tph1 (D) gene expression in mouse gut organoids after DBZ treatment. All the data were normalized by 18S. White, blue and red filled circles indicate mGO generated from FoxO1fl/fl, NFKO, VFKO mice, respectively. (E–N) Immunohistochemistry of FoxO1 (red) and 5HT (green). mGO were generated from FoxO1fl/fl (E, G, I, K, and M) or VFKO (F, H, J, L, and N), and analyzed before (E, F) and 24hr (G, H), 48hr (I, J), 72hr (K, L), and 96hr (M, N) after DBZ treatment. (O–R) GLP-1 (red) and 5HT (Green color). (S–V) C-peptide (red). mGO from FoxOfl/fl (O, Q, S, and U) or VFKO mice (P, R, T, and V) before (O, P, S, and T) and after (Q, R, U, and V) DBZ treatment for up to 96-hr. NFKO, Neurog3-Cre (+); FoxO1fl/fl: VFKO, Villin-Cre (+); FoxO1fl/fl. Scale bar = 50 μm. DAPI counterstains nuclei. Data are presented as means ± SD. ∗ = p < 0.05; ∗∗ = p < 0.01; ∗∗∗ = p < 0.001; ∗∗∗∗ = p < 0.0001.