Fig. 3.

Fecal transplantation with microbiota from patients with R-Y reconstruction relieves inflammation-driven colitis. (A–C) Weight change, stool consistency, and rectal bleeding score in mock, DSS control, R-Y FMT, B-I FMT, and B-II FMT mice following 2.5% DSS or H₂O treatment; n = 6. (D) Differences in the whole colon in each group. (E) Total colon length in mice in each group; n = 6. (F) Spleen weight in mice in each group; n = 6. (G) H&E staining and Alcian blue staining in each group. (H) Histological scores (based on the assessment of intestinal mucosa and submucosal glandular structure disorder, distortion and the absence of crypt structures, the degree of immune cell infiltration, and affected area); n = 6. (I) Kaplan–Meier survival analysis of DSS control and R-Y FMT mice treated with 3.5% DSS; n = 10. Scale bars = 50 μm. Error bars represent the mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.