F4/80+NOS2+ macrophages were decreased in R-Y FMT mice with DSS-treated colons. Representative images of immunofluorescence staining for (A) macrophage marker, F4/80 (green); M1 marker, NOS2 (red); and F4/80+NOS2+ cells (yellow, merge). (B) Macrophage marker, F4/80 (green); M2 marker, ARG1 (red); and F4/80+ARG1+ cells (yellow, merge). Cell nuclei, DAPI (blue). Representative merged images of at least three per group. Scale bars = 100 μm.