Fig. 2.

Crowding, cuboidal osteoblasts contribute to osteogenesis. (A) Histogram of osteoblast sphericity. (B) Osteoblasts shown with heatmap color and SHG of Days 10 and 14. (C) Division of sub‐ROIs in observation field of view. (D) Scatter plots of number of osteoblasts with high sphericity and increase in SHG. N = 208 from three independent experiments. R indicated Spearman's rank correlation coefficient. (E) Representative images of osteoblast density calculated by Delaunay triangulation. (F) Boxplot of mean areas of triangles. N = 13 from three independent experiments. One‐way ANOVA with Tukey's post hoc multiple comparison test. Scale bar: 25 μm.