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. 2022 Nov 10;35:100850. doi: 10.1016/j.neo.2022.100850

Fig. 4.

Fig. 4

IFIT1 altered PD-L1 protein expression. A. PCR analysis of PD-L1 mRNA levels in RKO cells after transfection with IFIT1 siRNAs (n=3, mean± SD, One-way ANOVA). B. Western blot assays detecting PD-L1 protein expression in RKO cells after transfection with IFIT1 siRNAs. C. PCR analysis of PD-L1 mRNA levels in RKO cells after transfection with pcDNA3.1_IFIT1 (n=3, mean± SD, two-tailed Student's t-test). D. Western blot assays detecting PD-L1 protein expression in RKO cells after transfection with pcDNA3.1_IFIT1. E. Immunofluorescence assay of PD-L1 in RKO cells with IFIT1 knockdown using siRNAs. Scale bar, 5µm. F. Flow cytometry detecting cell surface PD-L1 in IFIT1 knockdown RKO cells. G Statistic analysis of mean fluorescence intensity (MFI) of cell surface PD-L1 in IFIT1 knockdown RKO cells versus control RKO cells (n=3, mean± SD, two-tailed Student's t-test).

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