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. 2022 Nov 10;35:100850. doi: 10.1016/j.neo.2022.100850

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© 2022 Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — IFIT1 was methylated by METTL3 and METTL14 and recognized by IGF2BP2/3. A. PCR analysis of IFIT1 mRNA levels in HCT116 cells after transfection with METTL3 siRNAs (n=3, mean± SD, One-way ANOVA). B. PCR analysis of IFIT1 mRNA levels in HCT116 cells after transfection with METTL14 siRNAs (n=3, mean± SD, One-way ANOVA). C. Western blot assays detecting IFIT1 protein expression in HCT116 cells after transfection with METTL3 siRNAs. D. Western blot assays detecting IFIT1 protein expression in HCT116 cells after transfection with METTL14 siRNAs. E. PCR analysis of IFIT1 mRNA levels in HCT116 cells after transfection with pcDNA3.1_METTL3 and pcDNA3.1_METTL14 (n=3, mean± SD, two-tailed Student's t-test). F. Western blot assays detecting IFIT1 protein expression in HCT116 cells after transfection with pcDNA3.1_METTL3 and pcDNA3.1_METTL14. G. The detection of half-life(t1/2) of IFIT1 mRNAs in HCT116 cells transfected with control siRNAs and METTL3 siRNAs using real-time PCR, linear regression analysis. H. The detection of half-life(t1/2) of IFIT1 mRNAs in HCT116 cells transfected with control siRNAs and METTL14 siRNAs using real-time PCR, linear regression analysis. I. The detection of half-life(t1/2) of IFIT1 mRNAs in HCT116 cells with and without METTL3 overexpression using real-time PCR, linear regression analysis. J. The detection of half-life(t1/2) of IFIT1 mRNAs in HCT116 cells with and without METTL14 overexpression using real-time PCR, linear regression analysis. K. PCR analysis of IFIT1 mRNA level in HCT116 cells with IGF2BP1, IGF2BP2 and IGF2BP3 siRNAs transfection (n=3, mean± SEM, One-way ANOVA). L. The detection of half-life(t1/2) of IFIT1 mRNAs in HCT116 cells transfected with control siRNAs and IGF2BP2 siRNAs using real-time PCR, linear regression analysis. M. The detection of half-life(t1/2) of IFIT1 mRNAs in HCT116 cells transfected with control siRNAs and IGF2BP3 siRNAs using real-time PCR, linear regression analysis. N. The direct binding of IGF2BP2 with IFIT1 3’UTR using Agarose electrophoresis and RIP-qPCR assays in HCT116 cells (n=3, mean± SD, two-tailed Student's t-test). O. The direct binding of IGF2BP3 with IFIT1 3’UTR using Agarose electrophoresis and RIP-qPCR assays in HCT116 cells (n=3, mean± SD, two-tailed Student's t-test).

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