FIG. 1.

Wild-type V. cholerae N16961 (El Tor biotype) recovery from GI tracts of suckling mice over time. Five-day-old CD-1 mice were intragastrically inoculated with between 10⁵ and 10⁷ CFU as described in Material and Methods. Intestinal segments were aseptically removed and processed for bacterial quantification. (A) Percent CFU recovery from entire GI tract. For each time point, CFU from all segments were added and expressed as a percentage of the inoculum given to the mice at time zero. The averages and standard deviations of CFU recovered from four mice are shown. (B) Percent CFU recovery from small bowel. (C) Number of V. cholerae CFU recovered from indicated segments at various times postinoculation. The data are the averages from two mice from a single experiment in which the inoculum was 3 × 10⁵ CFU. Columns with asterisks above them were below the limit of detection for this assay (150 CFU).