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. 2022 Nov 14;21:210. doi: 10.1186/s12943-022-01675-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — ELOA mediates DLGAP1-AS2-driven CRC growth and metastasis. A-B Cell proliferation and colony formation abilities in WT and Trim21-KO HCT116 cells transfected with plasmids of DLGAP1-AS2 and ELOA. C Cell migration and invasion abilities in WT and Trim21-KO HCT116 cells transfected with plasmids of DLGAP1-AS2 and ELOA. D GSEA pathway enrichment analyses using the differentially expressed genes (DRGs) potentially affected by DLGAP1-AS2 and ELOA. DRGs were identified in DLGAP1-AS2-silenced and ELOA-overexpressing HCT116 cells using RNA-seq. E Validation of selected DRGs in HCT116 cells transfected with the siRNAs or plasmids of DLGAP1-AS2 and ELOA. F LHPP mRNA expression was detected by qRT-PCR in ELOA-overexpressing HCT116 cells. G The relative luciferase activity of LHPP promoter in ELOA-overexpressing HCT116 cells. H The recruitment of ELOA to the indicated regions of the LHPP promoter was determined by ChIP-PCR. IgG was used as the negative control. I The potential binding motif of ELOA was validated by luciferase assays. Mut: the mutant LHPP promoter lacking of the putative binding motif of ELOA. J Correlation analyses between DLGAP1-AS2 and LHPP. K The effect of DLGAP1-AS2/ELOA on the AKT pathway (pAKT at Ser473)