TABLE 2.

Primers used in this study

Primer name	Sequencea	Nucleotide recognition site (accession no.)
A start	5′-gga tcc aaG GAG ATA CAT ATA TGA AAA AAT GGA-3′	1885–1909 (U32175)
rev A end	5′-gtc gac TCG AAT GGC CAT CTT AGC ATC GAC-3′	5398–5421 (U32175)
88R	5′-aga tca aga tcT GCA ATG CAA GGT CGC CAA CAC-3′	2991–3010 (L23118)
88BFD	5′-aga tca aga tct CTG CCG CAC AGC TCC ATA GG-3′	4711–4730 (L23118)
5′BlueMCS	5′-gat gat cAG CGG ATA ACA ATT TCA CAC AGG-3′	827–846 (X52324)
3′BlueMCS	5′-gat gat cAC GCT TAC AAT TTC CAT TCG C-3′	451–470 (X52324)
NcoF	5′-ATG CAA ATG GTA TAA AAG TTA G-3′	5077–5099 (U32175)
A end Sal	5′-gtc gac CCA CTT ACA TCA ATC-3′	5576–5591 (U32175)

^aPrimer sequences homologous to the template are shown in capital letters, non-homologous sequences are in lowercase letters, and restriction sites incorporated into primers to facilitate the cloning of PCR products are underlined.