TABLE 3.
H. ducreyi hemolysin is cytotoxic to fibroblasts and human epithelial cells
| Strain | Normalized LDH releasea from:
|
||
|---|---|---|---|
| HFF | HFE | HEp-2 cells | |
| 35000 | 0.08 ± 0.00 | 0.20 ± 0.01 | 0.10 ± 0.01 |
| 35000ΔAPC | 0.02 ± 0.01 | 0.18 ± 0.03 | 0.05 ± 0.01 |
| 35000ΔAPC(pLSSK) | 0.02 ± 0.00 | 0.17 ± 0.01 | 0.05 ± 0.01 |
| 35000ΔAPC(pLSBA+2) | 19.25 ± 3.49 | 28.37 ± 8.69 | 2.74 ± 0.20 |
| 35000ΔAPC(pLS384-2) | 59.78 ± 2.85 | 88.70 ± 13.46 | 3.98 ± 0.25 |
| 35000ΔAPC(pLSB+) | 0.01 ± 0.00 | 0.14 ± 0.04 | 0.08 ± 0.04 |
| 35000ΔAPC(pLS376−) | 0.04 ± 0.00 | NDb | ND |
a
Values represent normalized LDH release (see Materials and Methods) and are the average of quadruplicate samples (HFFs were assayed in triplicate) ± standard error. The results of a typical experiment are shown; the experiment was repeated at least three times with similar results.
b
N.D., not determined.