TABLE 5.
H. ducreyi hemolysin is cytotoxic to human immune system cell lines
| Strain | Normalized LDH releasea from:
|
|||
|---|---|---|---|---|
| U937 cells | Jurkat cells | Daudi cells | Raji cells | |
| 35000 | 0.35 ± 0.05 | 1.08 ± 0.07 | 0.21 ± 0.01 | 0.12 ± 0.01 |
| 35000ΔAPC | 0.08 ± 0.02 | 0.07 ± 0.00 | 0.09 ± 0.02 | 0.08 ± 0.01 |
| 35000ΔAPC(pLSSK) | 0.18 ± 0.07 | 0.04 ± 0.01 | 0.06 ± 0.03 | 0.03 ± 0.02 |
| 35000ΔAPC(pLSBA+2) | 5.67 ± 0.30 | 73.63 ± 2.83 | 1.91 ± 0.18 | 1.16 ± 0.19 |
| 35000ΔAPC(pLS384-2) | 69.62 ± 10.42 | 225.95 ± 17.22 | 30.09 ± 0.12 | 4.23 ± 0.23 |
| 35000ΔAPC(pLSB+) | 0.17 ± 0.04 | 0.02 ± 0.05 | 0.06 ± 0.02 | 0.06 ± 0.02 |
a
Values represent normalized LDH release and are the average of quadruplicate samples ± standard error. The results of a typical experiment are shown; the experiment was repeated at least three times with similar results.