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The inhibitory effect of ABI-PP on the lower region mutants. (a) The growth of E. coli MG1655ΔacrBΔtolC cells harboring the plasmid pMMB67HE in the presence of 3 μg/mL of erythromycin and various concentrations of ABI-PP. (b to d) The growth of E. coli MG1655ΔacrBΔtolC expressing wild-type MexB (b), MexB(V139W) (c), and MexB(V571W) (d) in the presence of 3 μg/mL of erythromycin and various concentrations of ABI-PP. These tests were performed in triplicates. PP, ABI-PP inhibitor.
