FIG 5.

qRT-PCR results verifying RNA-seq and examining if any proteomics targets have altered transcript levels. Wild-type NZ131, Δrgg2, and Δrgg3 strains were grown in biological triplicate with or without 100 nM SHP peptide (indicated by + or – sign below each graph) and RNA was harvested late exponential phase. RNA was processed for qRT-PCR and transcript levels were determine relative to the gyrA reference gene. Significance of transcript level changes were determined using a one-way ANOVA with Multiple Comparisons Posttest. *, P < 0.05; **, P < 0.005; ****, P < 0.0005; ns, nonsignificant. For further experimental details see Materials and Methods. (A) Relative transcript levels of spy49_0450 (aroE.2). (B) Relative transcript levels of stcA (spy49_0414c). (C) Relative transcript levels of slo (spy49_0146). (D) Relative transcript levels of sfbX49 (spy49_1683c). (E) Relative transcript levels of upp (spy49_0322). (F) Relative transcript levels of spy49_0877.