FIG 9.

Examination of the impact of SHP induction and disruption of the Rgg2/3 system on nucleotide production. Thin layer chromatography (TLC) examining nucleotide levels in cells grown in MOPS-CDM with 150 μCi/mL ³²P-orthophosphate. As a control, an IPTG-inducible constitutive form of RelA from E. coli was run to provide standards for GTP, ppGpp, and pppGpp production; – indicates no IPTG added, + indicates 100 mM IPTG added. Wild-type NZ131 and derivative strains were grown with (+) or without (–) 100 nM SHP peptide. Labels beside the TLC indicate the location of GTP, ppGpp, and pppGpp as based on the IPTG induced E. coli pRelA* standard. This experiment was performed three times. Image shown was evenly adjusted for contrast and brightness.