TABLE 2.
Primers used in this study
| Primer | Purpose | Sequence (reference) |
|---|---|---|
| g70a_For | umuDAb E24K (HTH1) mutation | CGGCCGTAAAGCACAGTTCAATAAACCCACCAAAG |
| g70a_Rev | umuDAb E24K (HTH1) mutation | CTTTGGTGGGTTTATTGAACTGTGCTTTACGGCCG |
| a118c_a119c_a121c_a122c_For | umuDAb K40P N41P (HTH2) mutation | GACATTATTCAGTAACCAAGGTGGGATAAAGTTGACTTGAGATTCAGGAACACG |
| a118c_a119c_a121c_a122c_Rev | umuDAb K40P N41P (HTH2) mutation | CGTGTTCCTGAATCTCAAGTCAACTTTATCCCACCTTGGTTACTGAATAATGTC |
| c26aG27Tc28a | umuDAb A83Y mutation | CGCTGGTGATGGGAAACCATAAGCAACACGTTC |
| ASc26aG27Tc28a | umuDAb A83Y mutation | GCGACAGAACGTGTTGCTTATGGTTTCCCATCA |
| NotagNterm17978 | umuDAb amplification for cloning into pIX3.0 | AGAAGGAGATAAACAATGCCAAAGAAGAAAGAATTCGAG |
| NotagCterm17978 | umuDAb amplification for cloning into pIX3.0 | CTTGGTTAGTTAGTTATTATCTCATTCGTTTGAGGTTATAG |
| MoreOutFor | 17978 genomic region with umuDAb and ddrR | CGTTGAGTGTGTATGAATAG |
| MoreOutRev | 17978 genomic region with umuDAb and ddrR | CTTCTCAACCGCCTGTTTATC |
| Kmup | Amplify pCRII KanR cassette | CCGGAATTGCCAGCTGGG (56) |
| Kmdw | Amplify pCRII KanR cassette | TTCAGAAGAACTCGTCAAG (56) |
| KmupXho | Amplify pCRII KanR cassette on XhoI ends | AATAAGACTCGAGCCGGAATTGCCAGCTGGG |
| KmdwXho | Amplify pCRII KanR cassette on XhoI ends | AATAAGACTCGAGTCAGAAGAACTCGTCAAGAAG |
| 3662revXho | XhoI restriction site construction in p17M | AATAAGACTCGAGGTTTAAAATAATAAAAATAAAGGAG |
| ddrRrevXho | XhoI restriction site construction in p17M | AATAAGACTCGAGTTATGAGTGGGTAAGGG |
| A1S3606Forward | 17978 genomic region with A1S_1144 | GCTCTCCTTTTAGTGATGTAAC |
| A1S1144REV | 17978 genomic region with A1S_1144 | AACTAAGCCTGATGGACCAGAT |