TABLE 1.

Selection of ppe51 mutants with enhanced growth on glycerol at acidic pH

Plate no.	Mutant strain	SNP location	Quality score	Nucleotide change	Amino acid change in PPE51	Relative growth
1	eag1.7	3497961	5478	GCC→GAC	A228D	1.5
	eag1.8	3497911	6929	AGC→AGA	S211R	3.2
	eag1.12	3497961	7373	GCC→GAC	A228D	1.0
	eag1.14	3497911	6328	AGC→AGA	S211R	3.0
	eag1.33	3497911	5270	AGC→AGA	S211R	3.3
	eag1.34	3497911	5270	AGC→AGA	S211R	3.4
2	eag2.1	3497961	4417	GCC→GAC	A228D	2.0
	eag2.2	3497961	6803	GCC→GAC	A228D	2.5
	eag2.3	3497911	5205	AGC→AGA	S211R	3.8
	eag2.6	3497911	5976	AGC→AGA	S211R	2.7
	eag2.8	3497961	4270	GCC→GAC	A228D	1.6
	eag2.14	3497921	3442	GAG→AAG	E215K	0.9
	eag2.16	3497911	4575	AGC→AGG	S211R	3.6
3	eag3.2	3497961	4140	GCC→GAC	A228D	1.5
	eag3.4	3497961	3292	GCC→GAC	A228D	2.1
	eag3.9	3497911	3645	AGC→AGG	S211R	2.5
	eag3.15	3497911	3784	AGC→AGA	S211R	3.6
	eag3.23	3497911	4609	AGC→AGG	S211R	3.0
4	eag4.5	3497961	5153	GCC→GAC	A228D	1.7
	eag4.7	3497911	5107	AGC→AGG	S211R	2.5
	eag4.12	3497911	5179	AGC→AGG	S211R	1.2
	eag4.21	3497961	3958	GCC→GAC	A228D	1.9
	eag4.24	3497911	5517	AGC→AGG	S211R	2.5