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. 1999 Aug;67(8):3773–3779. doi: 10.1128/iai.67.8.3773-3779.1999

FIG. 3.

FIG. 3

The anti-Y. pseudotuberculosis CTL response in 21-4L rats is not restricted by HLA-B27. (A) LN cells from an 21-4L rat infected i.p. with Y. pseudotuberculosis IP2777(pYV+) were restimulated in vitro with 21-4L LN cells infected with IP2777(pYV+) and tested for lysis of IP2777(pYV+)-infected nontransgenic LEW (solid bars) or 21-4L (hatched bars) lymphoblast targets that were preincubated with saturating concentrations of anti-rat class I MHC (OX18), anti-HLA-B27 (B1.23.2), anti-rat class II MHC (OX6), or isotype-matched control MAb at an effector/target cell ratio of 100. Percent inhibition = (% lysis with control mAb − % lysis with relevant MAb)/% lysis without mAb × 100. (B) LN cells from 21-4L rat infected intragastrically with strain IP2777(pYV+) were restimulated in vitro with 21-4L LN cells infected with IP2777(pYV+) and tested for lysis of nontransgenic LEW (circles), 21-4L (squares), or RT1av1 21-4L backcross (DA B27+; triangles) rat lymphoblast targets infected with strain IP2777(pYV+) (closed symbols) or uninfected (open symbols).