Skip to main content
. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Nat Protoc. 2021 Oct 5;16(11):5339–5356. doi: 10.1038/s41596-021-00623-0

Fig. 1 |. Overview of SARS-CoV-2 spike structure and antigenicity.

Fig. 1 |

a, Schematic of the SARS-CoV-2 spike domains subdivided by color. NTD, N-terminal domain; RBD, receptor-binding domain; FP, fusion peptide; HR1, heptad repeat 1; CH, central helix; CD, connector domain. White region is unresolved in cryo-EM structures of the ectodomain. Glycan positions noted on top of the primary structure as previously reported63. Scissors denote the S1/S2 and S2′ proteolytic cleavage sites. Location of stabilizing HexaPro prolines is denoted. b,c, Spike structures in the three RBD down (PDB:6VXX) (b) and two RBD up prefusion states (PDB:6X2B) (c). Left: front view, Right: top view. HexaPro prolines shown in black. d, Composite structure of a spike monomer bound to neutralizing antibodies CR3022 (PDB: 6W41) and 4A8 (PDB:7C2L).