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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Nat Protoc. 2021 Oct 5;16(11):5339–5356. doi: 10.1038/s41596-021-00623-0

Fig. 2 |. Purification and characterization of the SARS-CoV-2 spike.

Fig. 2 |

a, Overview and timeline of the protocol. b, SDS-PAGE gel of recombinant spike after elution from the Strep-Tactin column. c, Superose-6 traces of S-2P and HexaPro. Note the higher yield for HexaPro. d, Differential scanning fluorimetry analysis. e, A negative-stain electron micrograph. f, 2D class averages of HexaPro.