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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Nat Protoc. 2021 Oct 5;16(11):5339–5356. doi: 10.1038/s41596-021-00623-0

Fig. 3 |. BLI analysis of spike-ACE2 binding.

Fig. 3 |

a, Schematic of the BLI experiment. Recombinant spikes with a C-terminal TwinStrep epitope are immobilized on a SA-coated tip. Association and dissociation of ACE2 is measured over time. b, Stepwise preparation of the BLI tip. Highest response is visualized with pre-binding spike and challenging with ACE2. c, Determination of the association (kon) and dissociation (koff) constants of spike for ACE2.