FIG. 4.

Analysis of immune response to M. catarrhalis total cellular proteins. The Tf receptor proteins from M. catarrhalis 4223 (lanes 2, 5, 8, and 11) or the Lf receptors from strain N141 (lanes 3, 6, 9, and 12) were isolated by using either hTf-Sepharose or hLf-Sepharose as the affinity matrix. The receptor proteins or strain N141 whole cells (lanes 1, 4, 7, and 10) were boiled in Laemmli sample buffer and subjected to SDS-PAGE (7% gel), and replicate Western blots were prepared. The blots were either stained for protein (lanes 1 to 3) or blocked and probed with either acute-phase sera from the patient infected with M. catarrhalis N141 (lanes 4 to 6), the convalescent-phase sera alone (lanes 7 to 9), or the convalescent-phase sera containing approximately 1 mg each of Mbp::TbpB and Mbp::LbpB purified by amylose affinity chromatography.