TABLE 1.
Gene | Regiona | Oligonucleotide sequenceb |
---|---|---|
tbpA | 5′ | TCTAGAATTCTTTCTTTGGGTCTGCTTAACATC |
tbpA | 3′ | CCCGGGAGATCTTTAAAACTTCATTTCAAGTGC |
tbpB | 5′ | TCTAGAATTGGTTCAGGTGGTTCAAATCCACC |
tbpB | 3′ | CCCGGGGGATCCTTACTTAACTTCTTGTTGTC |
lbpA | 5′ | AGAAGGGGTTCTGGTGTGGCAGTTTTACCCCTA |
lbpA | 3′ | TTAAGCTTAAAACTTCATTTCAAGACTGGC |
lbpB | 5′ | AAATCTAGACGCTCTGATGACATCAGCGTC |
lbpB | 3′ | TTAAGCTTATTTATCTTTAACAGCCCCAAAGAC |
5′, positive strand at the 5′ end of the gene; 3′, negative strand at the 3′ end of the gene.
Restriction endonuclease recognition sites are underlined.