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. 1999 Aug;67(8):3793–3799. doi: 10.1128/iai.67.8.3793-3799.1999

TABLE 1.

Oligonucleotide primer sequences used for PCR amplification

Gene Regiona Oligonucleotide sequenceb
tbpA 5′ TCTAGAATTCTTTCTTTGGGTCTGCTTAACATC
tbpA 3′ CCCGGGAGATCTTTAAAACTTCATTTCAAGTGC
tbpB 5′ TCTAGAATTGGTTCAGGTGGTTCAAATCCACC
tbpB 3′ CCCGGGGGATCCTTACTTAACTTCTTGTTGTC
lbpA 5′ AGAAGGGGTTCTGGTGTGGCAGTTTTACCCCTA
lbpA 3′ TTAAGCTTAAAACTTCATTTCAAGACTGGC
lbpB 5′ AAATCTAGACGCTCTGATGACATCAGCGTC
lbpB 3′ TTAAGCTTATTTATCTTTAACAGCCCCAAAGAC
a

5′, positive strand at the 5′ end of the gene; 3′, negative strand at the 3′ end of the gene. 

b

Restriction endonuclease recognition sites are underlined.