FIG. 3.

Preferential expansion of Vα2.3⁺ CD4⁺ T cells in the blast gate after a 6-day stimulation with live M. tuberculosis. The resting-cell gate (R1) and the blast gate (R2) were defined in forward-scatter (FSC), side-scatter (SSC) plots according to the population in the antigen-free cultures (A) and to large cells proliferating in response to live mycobacteria (B), respectively. The Vα2.3⁺ T-cell frequencies among CD4 cells were separately calculated for the resting-cell gate (C) and the blast gate (D).