Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (human) | A549 human lung epithelial cells; A549 | American Type Cell Collection | ATCC:CCL-185 | |
| Genetic reagent (bovine) | Calf thymus DNA; purified DNA | Invitrogen | ||
| Antibody | Rabbit polyclonal anti-histone H3 C-terminal; anti-H3-C; αH3-C | Active Motif | Active Motif:61277 | Western blot (1:15000) |
| Antibody | Rabbit polyclonal Histone H4 C-terminal; αH4-C | Abcam | Abcam:ab10158 | Western blot (1:5000) |
| Antibody | Rabbit monoclonal anti-histone H4 N terminal | Upstate Millipore, Sigma Aldrich | SigmaAldrich:05–858 | Western blot (1:30000) |
| Antibody | Rabbit monoclonal anti-GAPDH; GAPDH | Cell Signalling Technology | CST:2118 | Western blot (1:5000) |
| Antibody | Mouse monoclonal 3D9; 3D9: mouse anti-cleaved histone 3 3D9 | This paper | Custom synthesis by Genscript. Western blot (1 µg/ml), immunofluorescence (1–2 µg/ml) |
|
| Antibody | Rat monoclonal anti-histone H3 N-terminal; anti H3-N; αH3N | Active Motif | Active motif:61647 | Western blot (1:15000) |
| Antibody | Mouse monoclonal anti-H2A-H2B-DNA; PL2.3; anti-chromatin | PMID:1371530 | Immunofluorescence (1 µg/ml) | |
| Antibody | Rabbit polyclonal anti neutrophil elastase; anti-NE; αNE | Calbiochem | Calbiochem 481001 | Immunofluorescence (1:500) |
| Antibody | Rabbit polyclonal anti-histone H3 (citrullinine R2+R8+R17); anti- H3cit | Abcam | Abcam:ab5103 | Immunofluorescence (1 µg/ml) |
| Antibody | Chicken polyclonal anti-histone H2B | abcam | Abcam:ab134211 | Immunofluorescence (1:400) |
| Antibody | Polyclonal Sheep anti-ELANE: anti-NE | LSBio | LSBio:LS-B4244-50 | Immunofluorescence (5 µg/ml) |
| Antibody | Rabbit polyclonal Anti-mouse Bridging antibody | Active Motif | Active motif:53017 | (50 µg) |
| Antibody | Mouse monoclonal (1H7A8) IgG1, k, isotype control | Genscript | Provided by Genscript as an isotype control (1 µg/ml) | |
| Peptide, recombinant protein | Recombinant human histone H3.1 | New England Biolabs | NEB:M2503S | |
| Peptide, recombinant protein | Peptide H-REIRRK(RREIR)C-NH2; immunising peptide REIRR | This paper | Custom synthesis by Eurogentec | |
| Peptide, recombinant protein | Peptide H-AARKSK(SKRAA)C-NH2; negative screening peptide | This paper | Custom synthesis by Eurogentec | |
| Peptide, recombinant protein | Peptide H-REIRRK (RRIER_NH2; competition peptide) | This paper | Custom synthesis by Eurogentec | |
| Peptide, recombinant protein | Peptide H-TGGVKK(KVGGT)-NH2; negative control peptide | This paper | Custom synthesis by Eurogentec | |
| Commercial assay or kit | BD OptEIA TMB substrate reagent set | BD Biosciences | RRID:AB_2869044 | |
| Commercial assay or kit | Picogreen Assay kit; Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents | Thermofisher Scientific | Thermofisher:P7589 | |
| Commercial assay or kit | Cytoxicity assay; CytoTox 96 Non-Radioactive Cytotoxicity assay | Promega | Promega:G1780 | |
| Chemical compound, drug | Hoechst DNA stain (33342) | Invitrogen Molecular Probes | Invitrogen:H1399 | Immunofluorescence 1 µg/ml |
| Chemical compound, drug | Neutrophil elastase inhibitor GW311616A; NEi | Biomol | Biomol:Cay27957-1 | |
| Chemical compound, drug | AEBSF hydrochloride; peflabloc | Millipore, Sigma Aldrich | Sigma Aldrich:124839 | |
| Chemical compound, drug | Cathepsin G inhibitor I; CGi | Calbiochem, Sigma Aldrich | Sigma Aldrich:219372 | |
| Software, algorithm | Fiji; Image J | https://doi.org/10.1038/nmeth.2019 | ||
| Software, algorithm | NETalyser | This paper | (Scripts deposited at https://github.com/tulduro/NETalyser) | |
| Software, algorithm | Volocity | Perkin Elmer |