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. Author manuscript; available in PMC: 2023 Nov 7.
Published in final edited form as: Dev Cell. 2022 Nov 7;57(21):2497–2513.e6. doi: 10.1016/j.devcel.2022.10.003

Figure 6. Katanin is not regulated by α-tubulin Lys40 acetylation.

Figure 6.

(A) Severing of unmodified and acetylated microtubules. Yellow arrows, severing events. Scale bar, 5 μm.

(B) Normalized severing of unmodified and acetylated microtubules. Reactions at 20 nM katanin, 1 mM ATP; n = 89, 92 microtubules from multiple chambers for unmodified and acetylated, respectively.

(C) Normalized katanin levels bound to unmodified and acetylated microtubules. Binding performed at 4 nM katanin, 1 mM ATP; n = 38, 49 unmodified and acetylated microtubules, respectively, from multiple chambers. All error bars, S. D., p-value > 0.05 (ns) by Mann-Whitney test. LC-MS of microtubules and additional data in Figure S7.