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Immunoreactivity of the isoforms of the RgpA and RgpB proteases with MAb 1B5 HRgpA (lane 1), RgpA (lane 2), mt-RgpA (lane 3), RgpB (lane 4), and mt-RgpB (lane 5) (2 μg of each) were run on an SDS–12.5% polyacrylamide gel and stained with Coomassie blue (A) or Western blotted and reacted with MAb 1B5 (B).
