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. 2022 Oct 28;15(11):e011131. doi: 10.1161/CIRCEP.122.011131

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© 2022 The Authors.

Circulation: Arrhythmia and Electrophysiology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 1. — Effects of low- and intermediate-voltage pulsed electric fields on left ventricular myocyte shortening and intracellular Ca2+ ([Ca2+]i) at 1 minute after application. Representative sarcomere lengths and [Ca²⁺]_i traces obtained from an isolated left ventricular myocytes during baseline (control [Ctrl]) 1 Hz pacing and 1 minute after (T₀+1 minute) a 100 µs electric pulse (EP) delivered at low- (80 V; A) and intermediate-voltage (140 V; B). Sarcomere shortening, expressed as a percentage of the resting sarcomere length (%RSL) was increased 1 minute after EP with a low- and an intermediate-voltage pulses (C). Ca²⁺ transient (CaTr) amplitudes (D) and diastolic Ca²⁺ levels (E) expressed in 340:380 ratio units (r.u.), were increased 1 minute after EP with an intermediate but not with a low-voltage pulse. Data are represented as mean±SE of the mean with individual values for each cell. N indicates number of animals; n, number of cells; and ns, not significant. Paired t test: ns, **P<0.01, ***P<0.001.