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. 2022 Nov 15;209(10):1860–1869. doi: 10.4049/jimmunol.2200288

FIGURE 2.

FIGURE 2.

LCN2 and SAA are steroid-resistant IL-17A target genes. (A and B) hASMCs were untreated (CTRL) or treated for 24 h with human IL-17A (100 ng/ml), DEX (1 μM), or both. (C and D) mASMCs were untreated (CTRL) or treated with IL-17A (100 ng/ml), DEX (1 μM), or IL-17A+DEX for 24 h. The mRNA and protein levels were then analyzed by RT-PCR (A and C) and ELISA (B and D), respectively (n = 4 independent plates of cells). Data represent mean ± SEM. One-way ANOVA was performed, followed by Tukey’s multiple-comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.