Table 1.

MSI detection methods.

Method	Accuracy	Property tested
Fluorescent multiplexed PCR and capillary electrophoresis66	~100% (standard)	Instability in 5 distinct microsatellites
Next-generation sequencing67	~92–94%	Instability in ~100 distinct microsatellites
Single-molecule molecular inversion probes68	~90–95%	Instability in 111 distinct microsatellites
Immunohistochemistry69	~95% (standard)	Presence of MMR proteins