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. 2022 Nov 15;5:1253. doi: 10.1038/s42003-022-03981-x

Fig. 4. Drug effects on parasite number and gene expression in different parasite species and host cell types.

Fig. 4

a Parasite load per infected host cell was evaluated after drug treatment (2 µM for 48 h) compared to untreated (Ctrl), Buparvaquone (Bup) or DMSO-treated cells. Host cells (at least n = 50) were assessed for each experiment and parasite nuclei (DAPI staining) per host cell were counted manually. These results are representative of 3 independent experiments. Kruskal Wallis non-parametric test followed by Dunn’s multiple comparison test. ****p < 0.0001. Results are shown for T. annulata-infected macrophages (TaC12), B lymphocytes (TBL3) or T. parva-infected (TpM) lymphocytes. b Assessment of parasite viability using quantitative RT-qPCR analysis of parasite TaSP expression relative to bovine actin mRNA expression (host or parasite targeted first by the drug) in the three different cell lines compared to DMSO or Buparvaquone (Bup) treatment. Mean values ± SD. One-way Anova statistical test, Dunnett’s multiple comparison test: ****p < 0.0001.