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. 2022 Sep 19;16(12):2680–2690. doi: 10.1038/s41396-022-01322-8

Fig. 2. P. capeferrum F8 induces pyoluteorin biosynthesis in a distance-dependent manner.

Fig. 2

Concentration of DAPG (A) and pyoluteorin (B) was measured in two independent experiments measured with HR-LCMS. The control represents P. protegens DTU9.1 grown in isolation on KBmalt agar (set to 100 mm distance to fit the model). Dotted lines represent the 95% confidence intervals of the mathematical model. C Distance assay with P. protegens DTU9.1 harboring pSEVA237::PpltL-gfp indicating that increased pyoluteorin production was due to induced transcription of the pyoluteorin biosynthetic gene cluster in a distance-dependent manner. Pictures are representative of three biological replicates with similar results. Fluorescence images were obtained with 500 ms exposure time. D Relative fluorescence per colony was estimated with ImageJ. The dotted line represents the average fluorescence measured in the control cultivated axenically (Dunnett’s test compared to control: ***p < 0.001).